Volume 5, Issue 2 (6-2023)                   JAD 2023, 5(2): 57-62 | Back to browse issues page

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Ghaffari S M, Mahmoudi M, Salehi H, Sari A. Karyological analyses of four species of the families Lacertidae and Scincidae (Sauria) from Iran. JAD 2023; 5 (2) :57-62
URL: http://jad.lu.ac.ir/article-1-261-en.html
Karyological analyses of four species of the families Lacertidae and Scincidae (Sauria) from Iran
Seyed Mahmood Ghaffari 1, Musa Mahmoudi2 , Hasan Salehi3 , Alireza Sari2
1- Institute of Biochemistry and Biophysics, University of Tehran, Iran , mghaffary@ut.ac.ir
2- Department of Animal Biology, College of Science, University of Tehran, Iran
3- Department of Biology, Faculty of Science, Razi University, Kermanshah, Iran
Abstract:   (2753 Views)
Karyological studies were conducted using bone marrow cell preparations from four species of Lacertidae and Scincidae from Iran: Eremias persica Blanford, Eremias kopetdaghica Szczerbak, Ophisops elegans Ménétries (Lacertidae), and Eumeces schneiderii princeps (Eichwald) (Scincidae). Eremias persica was diploid with 2n = 38 chromosomes. The karyotype consisted of eighteen pairs of acrocentric macrochromosomes and two microchromosomes. Eremias kopetdaghica was also diploid with 2n = 38 comprising one pair of metacentric macrochromosomes, seventeen pairs of acrocentric macrochromosomes, and one pair of microchromosomes. The chromosome count for these two species is reported for the first time herein. The chromosome count for Ophisops elegans (2n = 38) was in agreement with a previous report and was the first for the herpetofauna of Iran. Eumeces schneiderii princeps showed 32 macro- and microchromosomes, representing the first report for this subspecies.


 
Keywords: Chromosome, Eremias, Eumeces schneiderii princeps, karyotype, lacertids, Ophisops elegans
Full-Text [PDF 1852 kb]   (596 Downloads)    
Type of Study: Short Communication | Subject: Molecular Diversity
Received: 2022/12/19 | Accepted: 2023/06/10 | Published: 2023/06/30
References
1. Adegoke, J. A. and Nadesan, S. (1986). Karyotype and polyploidy in the bone marrow of the African fruit bat, Eidolon belvum kerr. Nucleus, 29: 107-112.
2. Ananjeva, N. B., Orlov, N. L., Khalikov, R. G., Darevsky, I. S., Ryabov, S. A. and Barabanov, A. V. (2006). Persian Racerunner Eremias persica Blanford, 1874, In: The Reptiles of Northern Eurasia. Taxonomic Diversity, Distribution, Conservation Status. Pensoft Publishers, Sofia, Bulgaria. 98 pp.
3. Anderson, S. C. (1999). The lizards of Iran. Society for the Study of Amphibians and Reptiles, Ithaca, New York. 442 pp.
4. Arnold, E. N., Arribas, O. and Carranza, S. (2007). Systematics of the Palearctic and Oriental lizard Tribe Lacertini (Squamata: Lacertidae: Lacertinae), with descriptions of eight new genera. Zootaxa, 1430 (1): 1-86. [DOI:10.11646/zootaxa.1430.1.1]
5. Arronet (Kulikova), V. N. (1968). The karyotype of lizard, Ophisops elegans (Menetries). Tsitologiya, 10: 120-122.
6. Bahmani, Z., Rastegar-Pouyani, N., Rastegar-Pouyani, E. and Gharzi, A. (2014). A new record of Eremias strauchi strauchi Kessler, 1878 (Sauria: Lacertidae) from Kurdistan Province, Western Iran. Iranian Journal of Animal Biosystematics, 10 (1): 5-9.
7. Bhatnagar, A. N. and Yoniss, Y. Th. (1976). A proof of female heterogamety in a lacertid lizard, Ophisops elegans. Cytologia, 41: 307-511. [DOI:10.1508/cytologia.41.507]
8. Caputo, V., Odierna, G. and Aprea, G. (1994). A chromosomal study of Eumeces and Scincus, primitive members of the Scincidae (Reptilia, Squamata). Bollettino di Zoologia, 61 (2): 155-162. [DOI:10.1080/11250009409355876]
9. Dai, X., Zeng, X., Chen, B. and Wang, Y. (2004). The research on the karyotypes of six species in the genus Eremias from China. Hereditas, 26: 669-675. http://www.chinagene.cn/EN/Y2004/V26/I5/669
10. Eskandarzadeh, N., Rastegar-Pouyani, N., Rastegar-Pouyani, E., Fathinia, B., Bahmani, Z., Hamidi, K. and Gholamifard, A. (2018). Annotated checklist of the endemic Tetrapoda species of Iran. Zoosystema, 40 (sp1): 507-537. [DOI:10.5252/zoosystema2018v40a24]
11. Faizi, H., Rastegar-Pouyani, N., Rastegar-Pouyani, E. and Heidari, N. (2016). Systematics of the genera Eumeces Wiegmann, 1834 and Eurylepis Blyth 1854 (Sauria: Scincidae) in Iran: A review. Iranian Journal of Animal Biosystematics, 12 (2): 225-237.
12. Faizi, H., Rastegar-Pouyani, N., Rastegar-Pouyani, E., Nazarov, R., Heidari, N., Zangi, B., Orlova, V. and Poyarkov, N. (2017). A new species of Eumeces Wiegmann 1834 (Sauria: Scincidae) from Iran. Zootaxa, 4320 (2): 289-304. [DOI:10.11646/zootaxa.4320.2.5]
13. Firouz, E. (2000). A guide to the fauna of Iran. Iran University Press, Tehran, Iran. 491 pp. [In Persian]
14. Giovannotti, M., Caputo, V., O'Brien, P. C. M., Lovell, F. L., Trifonov, V., Nisi Cerioni, P., Olmo, E., Ferguson-Smith, M. A. and Rens, W. (2010). Skinks (Reptilia: Scincidae) have highly conserved karyotypes as revealed by chromosome painting. Cytogenetic and Genome Research, 127 (2-4): 224-231. [DOI:10.1159/000295002] [PMID]
15. Gorman, G. C. (1969). New chromosome data for 12 species of lacertid lizards. Journal of Herpetology, 3 (1/2): 49-54. [DOI:10.2307/1563223]
16. Greer, A. E. (1970). A subfamilial classification of scincid lizards. Bulletin of the Museum of Comparative Zoology at Harvard College, 139 (3): 151-183.
17. Greer, A. E. and Shea, G. M. (2000). A major new head scale character in non-lygosomine scincid lizards. Journal of Herpetology, 34 (4): 629-634. [DOI:10.2307/1565286]
18. Hosseinian Yousefkhani, S. S., Rastegar-Pouyani, E. and Aliabadian, M. (2016). Ecological niche differentiation and taxonomic distinction between Eremias strauchi strauchi and Eremias strauchi kopetdaghica (Squamata: Lacertidae) on the Iranian Plateau based on ecological niche modeling. Italian Journal of Zoology, 83 (3): 408-416. [DOI:10.1080/11250003.2016.1209581]
19. Hosseinian Yousefkhani, S. S., Yousefi, M., Rastegar-Pouyani, E. and Khani, A. (2013). Some remarks on the distribution and habitat preferences of the Eremias strauchi kopetdaghica Szczerbak (1972) (Sauria: Lacertidae) from the northeastern Iranian Plateau. Herpetology Notes, 6: 97-99.
20. Kaewsri, S., Yodmuang, S., Tanomtong, A., Patawang, I., Jumrusthanasan, S. and Pinthong, K. (2014). Cytogenetics of the Skinks (Reptilia, Scincidae) from Thailand; I: Chromosome analyses of the Common sun skink (Eutropis multifasciata). Cytologia, 79 (4): 457-466. [DOI:10.1508/cytologia.79.457]
21. Kostmann, A., Augstenová, B., Frynta, D., Kratochvíl, L. and Rovatsos, M. (2021). Cytogenetically elusive sex chromosomes in scincoidean lizards. International Journal of Molecular Sciences. 22: 8670. [DOI:10.3390/ijms22168670] [PMID] [PMCID]
22. Kyriazi, P., Poulakakis, N., Parmakelis, A., Crochet, P. A., Moravec, J., Rastegar-Pouyani, N., Tsigenopoulos, C. S., Magoulas, A., Mylonas, M. and Lymberakis, P. (2008). Mitochondrial DNA reveals the genealogical history of the snake-eyed lizards (Ophisops elegans and O. occidentalis) (Sauria: Lacertidae). Molecular Phylogenetics and Evolution, 49: 795-805. [DOI:10.1016/j.ympev.2008.08.021] [PMID]
23. Levan, A., Fredga, K. and Sandberg, A. A. (1964). Nomenclature for centromeric position on chromosomes. Hereditas, 52: 201-220. [DOI:10.1111/j.1601-5223.1964.tb01953.x]
24. Lisachov, A. P.,Giovannotti, M. B, Pereira, J. C., Andreyushkova, D. A., Romanenko, S. A., Ferguson-Smith, M. A., Borodin, P. M. and Trifonov, V. A. (2020). Chromosome painting does not support a sex chromosome turnover in Lacerta agilis Linnaeus, 1758. Cytogenetic Genome Research, 160: 134-140. [DOI:10.1159/000506321] [PMID]
25. Mozaffari, O. and Parham, J. F. (2007). A new species of racerunner lizard (Lacertidae: Eremias) from Iran. Proceeding of California Academy of Sciences, Series 4, 58 (28): 569-574.
26. Mozaffari, O., Ahmadzadeh, F. and Saberi-Pirooz, R. (2020). Fahimi's racerunner, a new species of the genus Eremias Fitzinger, 1834 (Sauria: Lacertidae) from Iran. Zootaxa, 4768 (4): 565-578. [DOI:10.11646/zootaxa.4768.4.7] [PMID]
27. Nasrabadi, R., Rastegar-Pouyani, N., Rastegar-Pouyani, E. and Gharzi, A. (2017). A revised key to the lizards of Iran (Reptilia: Squamata: Lacertilia). Zootaxa, 4227 (3): 431-443. [DOI:10.11646/zootaxa.4227.3.9] [PMID]
28. Odierna, G., Kupriyanova, L., Capriglione, T. and Olmo, E. (1993). Further data on sex chromosomes of Lacertidae and a hypothesis on their evolutionary trend. Amphibia-Reptilia, 14 (1): 1-11. [DOI:10.1163/156853893X00147]
29. Olmo, E. and Signorino, G. (2005). Chromorep: a reptile chromosomes database. Retrieved from: [DOI:10.1017/CBO9780511488900.009]
30. Oraie, H., Rahimian, H., Rastegar-Pouyani, N., Rastegar-Pouyani, E. and Khosravani, A. (2012). The easternmost record of Ophisops elegans (Sauria: Lacertidae) in Iran. Herpetology Notes, 5: 469-470.
31. Patawang, I., Tanomtong, A., Jumrusthanasan, S., Khongcharoensuk, H., Kaewsri, S. and Pinthong, K. (2017). Cytogenetic of Skink (Reptilia, Scincidae) from Thailand: II: Chromosome analyses of stripe tree skink (Lipinia vittigera). Cytologia, 82 (1): 83-90. [DOI:10.1508/cytologia.82.83]
32. Peccinini-Seale, D. (1981). New developments in vertebrate cytotaxonomy IV. Cytogenetic studies in reptiles. Genetica, 56: 123-148. [DOI:10.1007/BF00055413]
33. Rastegar-Pouyani, E., Hosseinian Yousefkhani, S. S. and Wink, M. (2015). Taxonomic reevaluation of Eremias strauchi strauchi Kessler, 1878 and Eremias strauchi kopetdaghica Szczerbak, 1972, based on nuclear and mitochondrial DNA sequences (Reptilia: Lacertidae). Zoology in the Middle East, 61 (2): 118-124. [DOI:10.1080/09397140.2015.1020615]
34. Rastegar-Pouyani, E., Hosseinian-Yousefkhani, S. S., Rafiee, S., Kami, H. G., Rajabizadeh, M. and Wink, M. (2016). A new species of the genus Eremias Fitzinger, 1834 (Squamata: Lacertidae) from Central Iran, supported by mtDNA sequences and morphology. Zootaxa, 4132 (2): 207-220. [DOI:10.11646/zootaxa.4132.2.2] [PMID]
35. Rastegar-Pouyani, E., Rastegar-Pouyani, N., Kazemi Noureini, S., Joger, U. and Wink, M. (2010). Molecular phylogeny of the Eremias persica complex of the Iranian Plateau (Reptilia: Lacertidae), based on mtDNA sequences. Zoological Journal of the Linnean Society, 158 (3): 641-660. [DOI:10.1111/j.1096-3642.2009.00553.x]
36. Singh, A. K. and Banerjee, R. (2004). Chromosomal diversity of Indian Mammals, Amphibians and Reptiles. Records of the Zoological Survey of India, 102 (3-4): 127-138. [DOI:10.26515/rzsi/v102/i3-4/2004/159516]
37. Šmíd, J., Moravec, J., Kodym, P., Kratochvíl, L., Hosseinian Yousefkhani, S. S. and Frynta, D. (2014). Annotated checklist and distribution of the lizards of Iran. Zootaxa, 3855: 001-097. [DOI:10.11646/zootaxa.3855.1.1] [PMID]
38. Taylor, E. H. 1935 (1936). A taxonomic study of the cosmopolitan scincoid lizards of the genus Eumeces, with an account of the distribution and relationships of its species. University of Kansas Science Bulletin, 23 (1): 1-642.
39. Uetz, P., Freed, P., Aguilar, R., Reyes, F. and Hošek, J. (Eds.) (2023) The Reptile Database, http://www.reptile-database.org Accessed [6 June 2023]
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