Journal of Animal Diversity

Journal of Animal Diversity

Phylogenetic analysis of a region of mitochondrial cox-1 as a DNA barcode marker sequence for the Siberian ibex Capra sibirica (Bovidae: Mammalia) in Mongolia

Document Type : Short Communication

Authors
Bayarlkhagva Damdingiin 1
Bolortuya Ulziibat 2
Bayarmaa Gun-Aajav 3
Davaa Bazarsad 4
Munkhjargal Bayarlkhagva 5
Enkhbaatar Batmagnai 6
1 Department of Biomedicine, Etugen University, Ulaanbaatar, Mongolia
2 Plant Biotechnology Laboratory, Institute of Biology, Mongolian Academy of Sciences, Ulaanbaatar, Mongolia
3 Department of Biology, School of Arts and Sciences, National University of Mongolia, Ulaanbaatar, Mongolia
4 Department of Scientific Analysis, National Institute of Forensic Science, Ulaanbaatar, Mongolia 5Zoological
5 Zoological Society of London’s Representative Office in Mongolia, Ulaanbaatar, Mongolia
6 Research Institute of Veterinary Medicine, Ulaanbaatar, Mongolia
10.22034/JAD.2024.6.2.4
Abstract
Siberian ibex or Altain yangir, Capra sibirica Pallas from Central Asia is believed to be the most ancient species of the genus Capra. In Mongolia, it is distributed in the areas of the Mongolian Altai, Gobi-Altai, Dzungaria, Altai, Khan Khuhii, Khoridal Saridag, and Ulaan Taiga as well as in the desert and semi-desert steppe zones of Dundgobi and Dornogobi provinces. Capra sibirica, a near-threatened species, is affected by illegal hunting for meat and sport. The mitochondrial cytochrome c oxidase subunit 1 gene (cox-1) is used as a DNA marker to distinguish mammalian species for the investigation of illegal hunting. In this study, we sequenced a part of the cox-1 of eight Mongolian Capra sibirica individuals. Our DNA sequences were clustered in a clade of Capra which is distinct from other clades of mammalian species in the phylogenetic tree. Our findings suggest that the DNA sequences can be utilized for the investigation of illegal hunting.
Keywords
Altain yangir
Capra
DNA barcoding system
illegal hunting
near threatened species

Subjects

1. Bayarlkhagva, M., Ulziibat, B., Gun-Aajav, B., Bazarsad, D., Damdingiin, B. and Batmagnai, E. (2023). Phylogenetic analysis of a region of mitochondrial cox-1 as a DNA barcode marker sequence of Gazella subgutturosa (goitered gazelle) in Mongolia. Animal Biodiversity and Conservation, 46 (2): 213-217. [DOI:10.32800/abc.2023.46.0213]
2. Cai, Y. S., Zhang, L., Shen, F. J., Zhang, W. P., Hou, R., Yue, B. S., Li, J. and Zhang, Z. H. (2011). DNA barcoding of 18 species of Bovidae. Chinese Science Bulletin, 56 (2): 164-168. [DOI:10.1007/s11434-010-4302-1]
3. Chen, J., Jiang, Z., Li, C., Ping, X., Cui, S., Tang, S., Chu, H. and Liu, B. (2015). Identification of ungulates used in a traditional Chinese medicine with DNA barcoding technology. Ecology and Evolution, 5 (9): 1818-1825. [DOI:10.1002/ece3.1457] [PMID] []
4. Chen, S. Y., Huang, C. C., Cheng, Y. T., Wang, C. C., Li, C. Y., Lai, I. L. and Hung, K. H. (2023). Effect of geographic isolation on genetic variation and population structure of Euphrasia nankotaizanensis, a threatened endemic alpine herb in Taiwan. Heliyon, 9 (3): e14228. [DOI:10.1016/j.heliyon.2023.e14228] [PMID] []
5. Fedosenko, A. K. and Blank, D. A. (2001). Capra sibirica. Mammalian Species, 675: 1-13. https://doi.org/10.1644/1545-1410(2001)675<0001:CS>2.0.CO;2 [DOI:10.1644/1545-1410(2001)6752.0.CO;2]
6. Ivanova, N. V., deWaard, J. R. and Hebert, P. D. N., (2006). An inexpensive, automation-friendly protocol for recovering high-quality DNA. Molecular Ecology Notes, 6 (4): 998-1002. [DOI:10.1111/j.1471-8286.2006.01428.x]
7. Lkhagvasuren, B. (2010). Distribution and resource assessment of Mongolian argal sheep (Ovis ammon L., 1758) and Sibirian ibex (Capra sibirica L., 1758): 114.
8. Mallon, D. P. (1985). The mammals of the Mongolian People's Republic. Mammal Review, 15 (2): 71-102. [DOI:10.1111/j.1365-2907.1985.tb00389.x]
9. Pidancier, N., Jordan, S., Luikart, G. and Taberlet, P. (2006). Evolutionary history of the genus Capra (Mammalia, Artiodactyla): Discordance between mitochondrial DNA and Y-chromosome phylogenies. Molecular Phylogenetics and Evolution, 40 (3): 739-749. [DOI:10.1016/j.ympev.2006.04.002] [PMID]
10. Nomura, K., Yonezawa, T., Mano, S., Kawakami, S., Shedlock, A. M., Hasegawa, M. and Amano, T. (2013). Domestication process of the Goat revealed by an analysis of the nearly complete mitochondrial protein-encoding genes. PLoS ONE, 8 (8): e67775. [DOI:10.1371/journal.pone.0067775] [PMID] []
11. Ramón-Laca, A., Gleeson, D., Yockney, I., Perry, M., Nugent, G. and Forsyth, D. M. (2014). Reliable discrimination of 10 ungulate species using high resolution melting analysis of Faecal DNA. PLoS ONE, 9 (3): 1-8. [DOI:10.1371/journal.pone.0092043] [PMID] []
12. Reading, R., Michel, S., Suryawanshi, K., and Bhatnagar, Y. V. (2020). Capra sibirica (Siberian Ibex). IUCN Read List, 8235: e.T42398A22148720.
13. Rodrigues, M. S., Morelli, K. A. and Jansen, A. M. (2017). Cytochrome c oxidase subunit 1 gene as a DNA barcode for discriminating Trypanosoma cruzi DTUs and closely related species. Parasites and Vectors, 10 (1): 1-18. [DOI:10.1186/s13071-017-2457-1] [PMID] []
14. Thiemann, T. C., Brault, A. C., Ernest, H. B. and Reisen, W. K. (2012). Development of a high-throughput microsphere-based molecular assay to identify 15 common bloodmeal hosts of Culex mosquitoes. Molecular Ecology Resources, 12 (2): 238-246. [DOI:10.1111/j.1755-0998.2011.03093.x] [PMID] []
Journal of Animal Diversity
Volume 6, Issue 2 - Serial Number 2
Cover photo: Hamadruas sp. (Araneae: Oxyopidae), from Bannur, Dakshina Kannada district, Karnataka, India. Photo by Shraddha Kumari K, June 2020.
Spring 2024
Pages 32-37